BASE-SPECIFIC SEPARATION OF OLIGODEOXYNUCLEOTIDES BY CAPILLARY AFFINITY GEL-ELECTROPHORESIS

Capillary affinity gel electrophoresis was applied to sequence-specifi c and base composition-specific recognition of oligodeoxynucleotides, utilizing the formation of heteroduplexes between a nucleic acid analo gue immobilized into the capillary gel and soluble oligodeoxynucleotid es with different sequences. Capillary affinity gel electrophoresis us ing capillaries filled with a conjugated gel of polyacrylamide and a s ynthetic nucleic acid analogue [poly(9-vinyladenine)] was effective fo r the selective separation of hexathymidylic acid from a mixture of fo ur homopolymers of A(6), C-6, G(6), and T-6 and for the complete resol ution of five heteropolymers of hexadeoxynucleotides (TAAAAA, TTAAAA, TTTAAA, TTTTAA, TTTTTA). We also demonstrated that capillary affinity gel electrophoresis was useful for the selective and the sensitive seq uence-specific recognition of sequence isomers of DNA (TTTTAA, TTTTAAT , TTTATA, and TTTTAA).