PHYLOGENETIC POSITION OF RARE HUMAN MYCOPLASMAS, MYCOPLASMA-FAUCIUM, MYCOPLASMA-BUCCALE, MYCOPLASMA-PRIMATUM AND MYCOPLASMA-SPERMATOPHILUM,BASED ON 16S RIBOSOMAL-RNA GENE-SEQUENCES
G. Rawadi et al., PHYLOGENETIC POSITION OF RARE HUMAN MYCOPLASMAS, MYCOPLASMA-FAUCIUM, MYCOPLASMA-BUCCALE, MYCOPLASMA-PRIMATUM AND MYCOPLASMA-SPERMATOPHILUM,BASED ON 16S RIBOSOMAL-RNA GENE-SEQUENCES, International journal of systematic bacteriology, 48(1), 1998, pp. 305-309
The nucleotide sequence of the 16S rRNA genes of four rare human mycop
lasma species, Mycoplasma faucium, M. buccale, M. primatum and M. sper
matophilum, were partially sequenced and compared to published rRNA ge
nes of mycoplasmas to determine their position in the Mollicutes phylo
genetic tree. Nucleotide sequence motif and overall similarities allow
ed positioning of these mycoplasmas in the hominis phylogenetic group,
as defined by Weisburg et al. [Weisburg, W. G., Tully, J, G., Rose, D
. L, & 9 other authors (1989). J Bacteriol 171, 6455-6467]. Furthermor
e, these mycoplasmas could be clustered into two different subdivision
s of the hominis group: (i) M. faucium and M. buccale were found to be
included in the M. fermentans subdivision, and (ii) M. primatum and M
. spermatophilum were included in the M. hominis one. Variable regions
of the 16S rRNA genes were used to determine specific PCR primers to
detect and identify M. faucium.