Ku. Gotz et G. Thaller, ASSIGNMENT OF INDIVIDUALS TO POPULATIONS USING MICROSATELLITES, Journal of animal breeding and genetics, 115(1), 1998, pp. 53-61
The article presents two methods for the assignment of an anonymous DN
A-sample re populations with, known allele frequencies at a number oi
marker loci. The first method calculates the conditional probability f
or the sample Eo belong to one of the two possible populations given t
he complete marker genotype at several loci. The second method is a si
mple test for thr sample to belong to a known population and is based
on the distribution of complete marker genotype probabilities. Simulat
ion studies were performed for the first method in order to estimate t
he expected probability for two populations with random allele frequen
cy differences. The results indicate that sis loci with four to five a
lleles each are sufficient to obtain an expected probability of more t
han 0.05 in 98.1 and 99.8 % of all possible situations. The simulation
studies for the second method were used to estimate the power of the
test for three different scenarios. The results show char with six loc
i with five alleles per locus a power between 0.90 and 0.98 can be ach
ieved. However, this is only true if the distribution of allele freque
ncies in the known population is not extremely skewed.