OPTICAL MEASUREMENT OF ISOLATED CANINE LUNG FILTRATION COEFFICIENTS AFTER ALLOXAN INFUSION

Citation
Jw. Klaesner et al., OPTICAL MEASUREMENT OF ISOLATED CANINE LUNG FILTRATION COEFFICIENTS AFTER ALLOXAN INFUSION, Journal of applied physiology, 84(4), 1998, pp. 1381-1387
Citations number
26
Categorie Soggetti
Physiology,"Sport Sciences
ISSN journal
87507587
Volume
84
Issue
4
Year of publication
1998
Pages
1381 - 1387
Database
ISI
SICI code
8750-7587(1998)84:4<1381:OMOICL>2.0.ZU;2-K
Abstract
In this study, lung filtration coefficient (Kf,) was measured in eight isolated canine lung preparations by using three methods: standard gr avimetric (Std), blood-corrected gravimetric (BC), and optical. The lu ngs were held in zone III conditions and were subjected to an average venous pressure increase of 8.79 +/- 0.93 (mean +/- SD) cmH(2)O. The p ermeability of the lungs was increased with an infusion of alloxan (75 mg/kg). The resulting K-fc values (in milliliters.min(-1) . cmH(2)O(- 1).100 g dry lung weight(-1)) measured by using Std and BC gravimetric techniques before vs. after alloxan infusion were statistically diffe rent: Std, 0.527 +/- 0.290 vs. 1.966 +/- 0.283; BC, 0.313 +/- 0.290 vs . 1.384 +/- 0.290. However, the optical technique did not show any sta tistical difference between pre-and postinjury with alloxan, 0.280 +/- 0.305 vs. 0.483 +/- 0.297, respectively. The alloxan injury, quantifi ed by using multiple-indicator techniques, showed an increase in perme ability and a corresponding decrease in rejection coefficient for albu min (sigma(f)). Because the optical method measures the product of K-f c and sigma(f), this study shows that albumin should not be used as an intravascular optical filtration marker when permeability is elevated . However, the optical technique, along with another means of measurin g K-fc (such as BC), can be used to calculate the sigma(f) of a tracer (in this study, sigma(f) of 0.894 at baseline and 0.348 after injury) . Another important finding of this study was that the ratio of baseli ne-to-injury K-fc values was not statistically different for Std and B C techniques, indicating that the percent contribution of slow blood-v olume increases does not change because of injury.