SOLUBLE THROMBOMODULIN AND ANTIBODIES TO BOVINE GLOMERULAR ENDOTHELIAL-CELLS IN PATIENTS WITH HENOCH-SCHONLEIN PURPURA

Citation
M. Fujieda et al., SOLUBLE THROMBOMODULIN AND ANTIBODIES TO BOVINE GLOMERULAR ENDOTHELIAL-CELLS IN PATIENTS WITH HENOCH-SCHONLEIN PURPURA, Archives of Disease in Childhood, 78(3), 1998, pp. 240-244
Citations number
29
Categorie Soggetti
Pediatrics
ISSN journal
00039888
Volume
78
Issue
3
Year of publication
1998
Pages
240 - 244
Database
ISI
SICI code
0003-9888(1998)78:3<240:STAATB>2.0.ZU;2-Z
Abstract
Aim-To evaluate the clinical significance of soluble thrombomodulin an d antiendothelial cell antibodies (AECA) in children with Henoch-Schon lein purpura. Methods-Binding of serum AECA to bovine glomerular endot helial cells was evaluated by enzyme linked immunosorbent assay, cytot oxicity against glomerular endothelial cells by spectrophotometric ass ay, and soluble thrombomodulin concentrations by sandwich enzyme immun oassay. Results-IgA AECA were detected in seven of 15 patients with He noch-Schonlein purpura and nephritis, but were not detected inpatients without nephritis or in controls. Patients with Henoch-Schonlein neph ritis had raised titres of IgA AECA and serum thrombomodulin; severe p roteinuria and renal histological changes were associated with raised titres of IgA AECA and raised serum thrombomodulin. No subjects had co mplement dependent cytotoxicity against glomerular endothelial cells. Conclusions-High titres of IgA AECA and raised serum thrombomodulin ma y be clinically useful markers of renal involvement in patients with H enoch-Schonlein purpura.