3-BROMOMETHYL-PROPYPHENAZONE AS A NEW DERIVATIZATION REAGENT FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF CAPTOPRIL AND HYDROCHLOROTHIAZIDE WITH UV-DETECTION

3-Bromomethyl-propyphenazone (BMP) was used as a derivatization reagen t for the detection and quantification of captopril (CAP) and hydrochl orothiazide (HCT) by high performance liquid chromatography using Zorb ax C8 column, and 0.05M sodium acetate, acetonitrile, methanol (14:17: 4; pH 6.5) as mobile phase system with UV-detection at 254 nm, The cit ed reagent reacts with the mercapto and amino groups of CAP and HCT in acetone using anhydrous potassium carbonate as hydrobromide acceptor. The reaction was completed within 30 min for CAP and 60 min for HCT w ith heating at 105+/-5 degrees C in mini-reaction vial. The linear con centration ranges for both CAP and HCT were 8 to 160 and 6 to 140 ng p er injection, respectively, The derivatized captopril was synthesized and confirmed with spectral analysis, This method was applied for dete rmination of spiked captopril in human urine after extraction with Ext relut-20 column using ethyl acetate:isopropanol (85:15 v/v) as eluant. (C) 1998 John Wiley & Sons, Ltd.