QUANTITATIVE STRUCTURE-RETENTION RELATIONSHIP STUDY OF TETRAZOLIUM SALTS ON ALUMINA SUPPORT

The retention of 7 monotetrazolium and 9 ditetrazolium salts was deter mined on alumina and reversed-phase (RP) alumina layers using n-hexane -2-propanol and water-2-propanol mixtures as eluents. The retention ca pacity and the specific surface area of solutes in contact with the st ationary phases were calculated. Quantitative structure-retention rela tionship calculations indicated that the retention capacity of solutes on RP alumina layers depended not only on the molecular hydrophobicit y but also on the hydrogen-donor and acceptor properties. Specific sur face areas were related to the electronic and steric parameters of the solutes. Calculations suggested that the retention on both alumina an d RP alumina layers is of mixed character, hydrophobic, electronic and steric parameters are equally involved in the retention. (C) 1998 Joh n Wiley & Sons, Ltd.