CYCLODEXTRIN DERIVATIVES IN GC SEPARATION OF RACEMIC MIXTURES OF VOLATILES - PART XIV - SOME APPLICATIONS OF THICK-FILM WIDE-BORE COLUMNS TO ENANTIOMER GC MICROPREPARATION

Representative examples of preparative GC isolation of pure enantiomer s in amounts ranging from 0.5 to 2 mg, with thick-film wide-bore open tubular columns coated with cyclodextrin (CD) diluted in polysiloxanes , are described, Two 25 m x 0.53 mm i.d. columns, coated with 3 mu m o f 30% 2,6-di-O-methyl-3-O-pentyl-beta-CD/OV-1701 and 2 mu m of 30% -ac etyl-6-O-tert-butyldimethylsilyl-beta-CD/PS-086 as stationary phases, were used, Methyl 3-hydroxyhexanoate, methyl 2-methylbutanoate, delta- hexalactone, delta-octalactone, and gamma-decalactone (the latter from a nature-identical mango aroma), were submitted to automated micropre parative GC. Average yields of about 30% were achieved when amounts ab ove 20 mu g/mu l were injected, and an enantiomeric purity above 90% w as obtained.