THERMODYNAMIC HYDROPHOBICITY OF AQUEOUS MIXTURES OF WATER-MISCIBLE ORGANIC-SOLVENTS PREDICTS PEROXIDASE-ACTIVITY

The effect of different water-miscible organic solvents on biocatalyti c activities of chloroperoxidase from Caldariomyces fumago and horsera dish peroxidase was determined. A new hydrophobicity parameter for wat er-organic solvent mixtures was used to predict the enzyme behavior. T his thermodynamic concept of hydrophobicity also describes the catalyt ic behavior of three other biocatalysts with peroxidase activity. So f ar, all reported data of peroxidase activity in increasing concentrati ons of water-miscible organic solvents are effectively predicted by th e thermodynamic model presented in this work. (C) 1998 Elsevier Scienc e B.V.