PHENYL VALERATE CARBOXYLESTERASES OF CHICK-EMBRYO BRAIN AS A MARKER FOR A NEW MICROASSAY OF PHOSPHOLIPASE A(2)

Authors
SEIFERT J WILSON BW
Citation
J. Seifert et Bw. Wilson, PHENYL VALERATE CARBOXYLESTERASES OF CHICK-EMBRYO BRAIN AS A MARKER FOR A NEW MICROASSAY OF PHOSPHOLIPASE A(2), Analytical letters, 31(4), 1998, pp. 601-611
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
31
Issue
4
Year of publication
1998
Pages
601 - 611
Database
ISI
SICI code
0003-2719(1998)31:4<601:PVCOCB>2.0.ZU;2-Z
Abstract
This paper reports the development of a new microassay for phospholipa se A(2) (PL A(2)) [EC 3.1.1.4] using the phenyl valerate carboxylester ases (PVCases) of chick embryo brain as a marker for PL A(2) catalytic activity. The activity of PVCases released from brain membrane fragme nts (BMF) by PL A(2) was determined spectrophotometrically by measurin g the phenol formed from phenyl valerate hydrolysis with potassium fer ricyanide and 4-aminoantipyrine. The enzymes most active on chicken em bryonic BMF were PL A(2) of Naja and bee venoms.