DETERMINATION OF CYCLOSPORINE-A BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ARYL OXALATE CHEMILUMINESCENCE DETECTION

Monitoring of cyclosporine A in 40 whole blood samples from renal tran splantation patients by high performance liquid chromatography with ar yl oxala,te-sulforhodamine 101 chemiluminescence detection is describe d. These data were compared with fluorescence polarized immunoassay (C yclosporine-SP Dynapack(TM) using monoclonal antibody for whole blood) , with good results (r = 0.88, y = 0.80 + 5.07). These values are the same as reported by high performance liquid chromatography with photom etric detection. By using a solid phase extraction cartridge, cyclospo rine A was analyzed within 30 min. The analysis time could be shortene d to one tenth that previously reported. The proposed method has the s ame sensitivity as fluorescence polarized immunoassay.