DIASTEREOMERIC ANALYSIS OF BIOACTIVE N-PHENYLPYRAZOLE-4-ACYLHYDRAZONEDERIVATIVES BY HIGH-RESOLUTION GAS-CHROMATOGRAPHY

Bioactive acylhydrazone samples prepared by condensation of 1-phenyl-4 -acylhydrazide pyrazole with benzaldehyde were analyzed by H-1 NMR, ma ss spectrometry and High Resolution Gas Chromatography (HRGC), utilizi ng a glass capillary column coated with SE-54 (1%vinyl-5%phenyl-94%met hylpolysiloxane). HRGC was shown to be a valuable tool for the routine analysis of the acylhydrazones, and determination of the diastereomer ic ratio, without need of derivatization and purification. As an examp le, HRGC was employed to follow the kinetics of formation at the imino double bond level. of both diastereomers of hidrazone (1a) during a s ynthetic procedure.