THE EFFECT OF PROTEIN-KINASE-C INHIBITORS ON INVASION OF HUMAN OVARY CANCER-CELLS

In the present work we tested whether invasiveness of ovarian carcinom a cells could be considered as protein kinase C (PKC) dependent proces s. The migration and invasion studies were performed in Transwell cham bers. Staurosporine, sphingosine and tamoxifen were used as PKC inhibi tors. Also the effect of prolonged treatment with TPA was the subject of observation. The obtained results indicated that invasion understoo d as three step process (attachment, migration and matrix degradation) was affected by PKC inhibitors. The detailed studies, however, showed that attachment and matrix degradation ability of ovarian cancer cell s was not changed by PKC inhibitors as opposed to migration which was, at least partly, regulated by protein kinase C.