ONGOING PREGNANCIES AFTER INTRACYTOPLASMIC INJECTION USING CRYOPRESERVED TESTICULAR SPERMATOZOA

We report two clinical pregnancies occurring after intracytoplasmic sp erm injection (ICSI) using cryopreserved spermatozoa obtained from tes ticular biopsy, made in two different infertility situations in our cl inic, The first patient showed a secretory azoospermia associated with elevated serum follicular stimulating hormone (FSH) level and spermio genesis maturation arrest. The second patient was affected by azoosper mia resulting from bilateral epididymal obstruction, Spermatozoa prese nt in the wet preparation of testicular biopsy made on the day of scro tal exploration were cryopreserved within the testicular tissue for bo th men, Intracytoplasmic injections were performed at a later date, us ing spermatozoa prepared from frozen-thawed tissues, In each case, thr ee embryos were obtained and transferred in utero. The transfers resul ted in a twin pregnancy for the first case, and in a singleton pregnan cy for the second, Living foetuses were seen in the ultrasound scan at the 7th week and both pregnancies are proceeding to date beyond 30 we eks without complications.