S. Perraguinjayot et al., ONGOING PREGNANCIES AFTER INTRACYTOPLASMIC INJECTION USING CRYOPRESERVED TESTICULAR SPERMATOZOA, Human reproduction, 12(12), 1997, pp. 2706-2709
We report two clinical pregnancies occurring after intracytoplasmic sp
erm injection (ICSI) using cryopreserved spermatozoa obtained from tes
ticular biopsy, made in two different infertility situations in our cl
inic, The first patient showed a secretory azoospermia associated with
elevated serum follicular stimulating hormone (FSH) level and spermio
genesis maturation arrest. The second patient was affected by azoosper
mia resulting from bilateral epididymal obstruction, Spermatozoa prese
nt in the wet preparation of testicular biopsy made on the day of scro
tal exploration were cryopreserved within the testicular tissue for bo
th men, Intracytoplasmic injections were performed at a later date, us
ing spermatozoa prepared from frozen-thawed tissues, In each case, thr
ee embryos were obtained and transferred in utero. The transfers resul
ted in a twin pregnancy for the first case, and in a singleton pregnan
cy for the second, Living foetuses were seen in the ultrasound scan at
the 7th week and both pregnancies are proceeding to date beyond 30 we
eks without complications.