Zygotes morphologically classified as tripronuclear (3PN) after intrac
ytoplasmic sperm injection (ICSI), which are thought to be digynic in
their origin, were studied by fluorescent in-situ hybridization (FISH)
. FISH results allowed us to assess the suspected ploidy after morphol
ogical evaluation of the zygote and to determine the origin of the thi
rd pronuclei, Our results show that, firstly, 36% of those zygotes cla
ssified as 3PN following their morphological evaluation were, in fact,
diploid, and secondly, the main cause for triploidy after ICSI is the
non-extrusion of the second polar body.