S. Sasaki et al., ELASTIC PROPERTIES OF LIVING FIBROBLASTS AS IMAGED USING FORCE MODULATION MODE IN ATOMIC-FORCE MICROSCOPY, Archives of histology and cytology, 61(1), 1998, pp. 57-63
Using the force modulation mode in atomic force microscopy, we measure
d elastic properties of living mouse fibroblasts (NIH3T3) in a culture
medium, The topographic images of the cellular surface and the corres
ponding elastic images of the cellular surface were able to be capture
d simultaneously with high spatial resolution, The consecutive images
were useful for examining time-dependent changes in the cellular surfa
ce, We observed that some cells continued to shrink and change their s
oftness for 2 hours, Then the force modulation mode in atomic force mi
croscopy shows potential use in analyzing time-dependent regional elas
tic properties of living cells with high spatial resolution.