SOMATIC EMBRYOGENESIS AND PLANT-REGENERATION IN IMMATURE ZYGOTIC EMBRYO, OVULE, AND ANTHER FILAMENT CULTURES OF CHINESE-CABBAGE

Immature zygotic embryos, ovules, and anther filaments of Chinese cabb age were cultured on Murashige and Skoog's (MS) medium supplemented wi th 1 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Up to 80% of the zygotic embryos, 17% of the ovules, and 13% of the filaments produced somatic embryos with or without intervening calli. Upon transfer to M S basal medium, most of the somatic embryos developed into plantlets. Plantlets were transplanted to potting soil and grown in a phytotron. (C) 1998 Elsevier Science B.V.