SUPPLEMENTATION OF BIFIDOBACTERIUM-LONGUM TO A HIGH-FAT, LOW-CALCIUM DIET LOWERS CYTOLYTIC ACTIVITY OF FECAL WATER IN RATS INJECTED WITH 1,2-DIMETHYLHYDRAZINE DIHYDROCHLORIDE

The effects of supplementing Bifidobacterium longum SET 2928 and Lacto bacillus acidophilus SET 2062 to a high-fat, low-calcium diet on bile acid concentration, fatty acid concentration, cytolytic activity and i ntestinal alkaline phosphatase (ALP) activity of fecal water in rats i njected with and without 1,2-dimethylhydrazine dihydrochloride (DMH) w ere examined. Male Wistar rats at 8 weeks of age were fed a diet conta ining 18% coconut oil, 2% corn oil and 0.1% calcium for 15 d. Lyophili zed cultures were supplemented to test diets at a concentration of 1%. The feeding of a high-fat, low-calcium diet elevated the bile acid co ncentration, cytolytic activity and ALP activity of fecal water as com pared to the AIN-76A diet, whereas the fatty acid concentration was no t changed. None of the cultures had any effect on these parameters. Fu rthermore, 8 week-old rats were given a single subcutaneous injection of DMH at 40 mg/kg body weight, and fed the same diets for 15 d, The D MH injection had no effect on the bile acid concentration but increase d the fatty acid concentration and cytolytic activity of fecal water. In contrast, ALP activity was lower in the DMH-treated rats than in th e non-treated rats. The ingestion of B. longum lowered cytolytic activ ity but had no effect on the bile acids, fatty acids and ALP activity of fecal water. L. acidophilus had no effect on these parameters.