WHAT ARE THE PRACTICAL LIMITS FOR DETECTION OF MINOR NUCLEOSIDE REACTION-PRODUCTS WITH HPLC (UV DETECTION), 1H NMR, AND TLC (UV DETECTION)

Detection limits for the minor component in binary mixtures of Ado/Ara A, Ado/XyloA, and Urd/dUrd depend strongly on the combined concentrati on of analytes. Limiting concentrations (in which less than or equal t o 1% of the minor component was detected) were about two orders of mag nitude lower with HPLC (UV detection) than with H-1 NMR and TLC (UV de tection) with these nucleosides (epsilon(max) 10000-15000). Minimum mo lar percentages of minor components detected in the 0.1-10 mM range we re 0.25-1% with HPLC (UV), 1-2% with H-1 NMR, and similar to 2% with T LC (UV).