Fusicoccin-like ligands in higher plants were sought by combining high
performance liquid chromatography with radioreceptor analysis and rad
ioimmunoassay. Such substances were found in genetically transformed c
ultured roots of horseradish, alteus and lupine as well as in native h
orseradish, cucumber, horse chestnut, and maize plants. In root crops
such as carrot and sugar beet or in potato tubers, only traces of fusi
coccin-like ligands were detected. Fusicoccin A was detected in geneti
cally transformed cultured roots of horseradish by gas chromatography/
mass spectrometry.