NEW METAL CHELATE SORBENT FOR ALBUMIN ADSORPTION - CIBACRON BLUE F3GA-ZN(II) ATTACHED MICROPOROUS POLY(HEMA) MEMBRANES

Poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an ini tiator (alpha-alpha'-azobis-isobutyronitrile, AIBN). The triazine dye Cibacron Blue F3GA was attached as an affinity ligand to poly(HEMA) me mbranes, covalently. These affinity membranes with a swelling ratio of 58% and containing 10.7 mmol Cibacron Blue F3GA/m(2) were used in the albumin adsorption studies. After dye-attachment, Zn(TI) ions were ch elated within the membranes via attached-dye molecules. Different amou nts of Zn(II) ions [650-1440 mg Zn(II)/m(2)] were loaded on the membra nes by changing the initial concentration of Zn(II) ions and pH. Bovin e serum albumin (BSA) adsorption on these membranes from aqueous solut ions containing different amounts of BSA at different pH was investiga ted in batch reactors. The nonspecific adsorption of BSA on the poly(H EMA) membranes was negligible. Cibacron Blue F3GA attachment significa ntly increased the BSA adsorption up to 92.1 mg BSA/m(2). Adsorption c apacity was further increased when Zn(II) ions were attached (up to 11 4.8 mg BSA m(2)). More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 0.5M NaSCN at pH 8.0 and 0.025M EDTA at pH 4.9. (C) 1998 John Wiley & Sons, Inc.