Fluorescence microscopy has been adapted to cryogenic temperatures and
combined with efficient image processing and analysis to observe many
single molecules in parallel. The capabilities of this setup are demo
nstrated with the parallel recording of peak-frequency trajectories fo
r 62 terrylene-molecules in n-hexadecane and with the measurement of 9
18 single molecule linewidths for terrylene in dodecane in 200 s. (C)
1998 Elsevier Science B.V.