SIGNAL ENHANCEMENT IN CAPILLARY ELECTROPHORESIS BY USING A SLEEVE CELL ARRANGEMENT FOR OPTICAL-DETECTION

This study compares signal enhancement and efficiency in CZE employing three modes of detection: the sleeve cell (a simple method for creati ng a region of extended path length for absorption detection), the bub ble cell (extended light path capillaries), and on-column detection in 75 mu m i.d. capillary, Flow profile in the sleeve cell was monitored under a microscope, An abrupt change in capillary diameter in the sle eve cell region (from 50 mu m to 220 mu m) did not produce extensive b and broadening. The sleeve-cell detection arrangement delivered a 3.5 fold increase in corrected peak area when compared with an on-column d etection in 75 mu m i.d. column.