USE OF CATIONIC CYCLODEXTRIN FOR ENANTIOSEPARATION BY CAPILLARY ELECTROPHORESIS

The usability of hydroxy-3-trimethylammoniopropyl-beta-cyclodextrin fo r chiral discrimination of various basic and acidic substances is desc ribed. The dependence of chiral separation on cyclodextrin (CD) concen tration and pH value was investigated. Altering the pH value the migra tion order of the enantiomers of acidic analytes could be changed. Due to the quaternary ammonium structure of the CD molecule, a reversal o f the electroosmotic flow (EOF) was observed. The direction of the mig ration of CD molecule was in opposite direction to the EOF because of its positive charge. The influence of CD concentration and pH on the E OF was determined. (C) 1998 Elsevier Science B.V.