STUDY OF RETENTION IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY USING LINEAR SOLVATION ENERGY RELATIONSHIPS - II - THE MOBILE-PHASE

The applicability of linear solvation energy relationships (LSERs) to reversed-phase liquid chromatography (RPLC) was studied by examining t he retention of a wide variety of aliphatic and aromatic compounds ove r the range of 20-50% (v/v) acetonitrile, methanol and tetrahydrofuran . The role of cavity formation, dispersion interaction, polarity/polar izability, hydrogen bond acidity, and hydrogen bond basicity in determ ining the retention behavior as the mobile phase composition was chang ed has been investigated. The LSER coefficients were then examined in terms of the corresponding properties of the mobile phase (cohesive en ergy density, surface tension, the Abraham solvophobic parameter, pola rity/polarizability, hydrogen bond basicity, and hydrogen bond acidity ) and from these the influence of mobile phase and stationary phase on the retention behavior was explored, in order to chemically interpret the RPLC retention results we compared them to alkane-water and octan ol-water partition coefficients. (C) 1998 Elsevier Science B.V.