Om. Steijger et al., LIQUID-CHROMATOGRAPHY WITH LUMINOL-BASED ELECTROCHEMILUMINESCENCE DETECTION - DETERMINATION OF HISTAMINE, Journal of chromatography, 799(1-2), 1998, pp. 57-66
Citations number
38
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The liquid chromatographic determination of histamine was achieved by
precolumn derivatization with N-(4-aminobutyl)-N-ethylisoluminol isoth
iocyanate and electrochemiluminescence detection. Detection was carrie
d out using postcolumn on-line electrochemical reagent generation and
oxidation of the derivatized analyte at a gold gauze working electrode
built in the flow cell of the detector. With this on-line method a de
tection limit of 1.5 pmol (S/N=3) of histamine was obtained. The metho
d was linear in the range 0.5-10 nmol and the repeatability of the met
hod was satisfactory (R.S.D.=13.7% and 3.7% at the 0.5 and 5 nmol leve
l, respectively). (C) 1998 Elsevier Science B.V.