RAPID PCR FOR IDENTITY TESTING USING A BATTERY-POWERED MINIATURE THERMAL CYCLER

A microfabricated, battery-powered thermal cycler was implemented in P CR-based DNA typing for human identification. HLA DQ alpha and an STR tripler were PCR amplified using a device known as the Miniature Analy tical Thermal Cycling Instrument (MATCI). The extremely efficient heat ing properties of the MATCI enabled thermal cycling to be completed in as little as 21 min. In addition, the feasibility of using the real-t ime fluorescent detection system of the MATCI was demonstrated. The su ccessful application of this portable, prototype device to forensic id entity testing is a significant milestone towards the eventual develop ment of a completely integrated DNA testing instrument that would also incorporate sample preparation and allele detection.