S. Ozcan et al., PROLIFIC SHOOT REGENERATION FROM IMMATURE EMBRYO EXPLANTS OF SAINFOIN(ONOBRYCHIS-VICIIFOLIA SCOP), Plant cell reports, 16(3-4), 1996, pp. 200-203
Immature cotyledons and embryo axes of sainfoin were cultured on Muras
hige and Skoog (MS) media supplemented with various concentrations of
6-benzylaminopurine (BAP) and alpha-naphthaleneacetic acid (NAA) to in
duce adventitious shoot regeneration. The highest frequency of shoot r
egeneration occurred following an initial callus growth on a MS medium
containing 0.5 mg/l BAP and 2 mg/l NAA Immature embryo axes showed hi
gher regeneration capacity than immature cotyledons, however, shoot el
ongation was best achieved on immature cotyledons. Regenerated shoots
were excised and rooted in half strength MS medium with 1 mg/l indole-
butyric acid (IBA) or 1 mg/l NAA. The rooted plantlets were finally tr
ansferred to compost.