ACOUSTIC-WAVES AND THE REAL-TIME STUDY OF BIOCHEMICAL MACROMOLECULES AT THE LIQUID SOLID INTERFACE/

The adsorption of the proteins, bovine serum albumin, fibrinogen, avid in and neutravidin (non-glycosylated form of avidin) to a variety of s urfaces imposed on thickness shear mode sensors is examined in a flow- injection analysis format. In all cases, adsorption of these moieties was essentially irreversible, although the magnitude of adsorption was dependent on surface free energy and functional group chemistry. Also described is the direct, real-time detection of the binding of peptid es to HIV-1 TAR RNP, bound on a thickness-shear mode (TSM) sensor surf ace. The results clearly indicate that responses are discriminatory fo r two different peptides. In order to provide a theoretical backcloth for the experimental measurements, a new model for the operation of th e TSM in liquids is presented.