Dw. Siemann et al., RADIOBIOLOGICAL HYPOXIA IN THE KHT SARCOMA - PREDICTIONS USING THE EPPENDORF HISTOGRAPH, International journal of radiation oncology, biology, physics, 40(5), 1998, pp. 1171-1176
Citations number
35
Categorie Soggetti
Oncology,"Radiology,Nuclear Medicine & Medical Imaging
Purpose: To investigate whether electrode measurements of tumor oxygen
ation obtained under a range of different treatment conditions designe
d to alter the degree of tumor hypoxia could be correlated with estima
tes of radiobiological hypoxia measured under the same conditions. Met
hods and Materials: Experiments were performed in restrained, nonanest
hetized, female C3H/He mice, which had similar to 0.5 g KHT sarcomas g
rowing intramuscularly in the hind limbs, The treatments used to modif
y tumor oxygenation status included breathing gas mixtures of varying
oxygen content, altering tumor blood Bow, and shifting the hemoglobin
oxygen dissociation curve, Radiobiological hypoxic fraction was estima
ted using the paired survival curve assay, while electrode measurement
s of tumor oxygenation were obtained with an Eppendorf histograph. Res
ults: With the selected manipulations it was possible to vary the radi
obiological hypoxic fraction in the tumors from similar to 1 to simila
r to 100% of the total viable cell population, Furthermore, these chan
ges in radiation response were directly reflected in the changes in tu
mor oxygenation measurements made with the Eppendorf histograph, Concl
usion: These findings suggest that in the KHT tumor model the Eppendor
f electrode measurements could predict the response of the tumors to r
adiation as determined by the proportion of hypoxic cells. (C) 1998 El
sevier Science Inc.