T. Kalai et al., DOUBLE (FLUORESCENT AND SPIN) SENSORS FOR DETECTION OF REACTIVE OXYGEN SPECIES IN THE THYLAKOID MEMBRANE, Free radical biology & medicine, 24(4), 1998, pp. 649-652
A series of dansylated sterically hindered amines designed to trapping
reactive oxygen species, were synthesized. Compounds were tested in i
solated thylakoid membranes subjected to photoinhibition by excess pho
tosynthetically active radiation (400-700 nm). DanePy showed good sele
ctivity for singlet oxygen and the formation of nitroxide was detected
by appearance of ESR signal and quenching fluorescence. (C) 1998 Else
vier Science Inc.