DIFFERENTIAL-PULSE POLAROGRAPHIC-DETERMINATION OF OFLOXACIN IN PHARMACEUTICALS AND BIOLOGICAL-FLUIDS

A sensitive method is described for the determination of ofloxacin in its pure form, dosage forms and biological fluids. The proposed method depends upon the polarographic activity of ofloxacin in Britton Robin son buffers, whereby a well-defined cathodic wave is produced over the pH range 4.1-10.3. The wave was characterized as being irreversible, diffusion-controlled with limited adsorption properties. The current-c oncentration relationship was found to be rectilinear over the range 5 x 10(-5)-5 x 10(-4) M and 1 x 10(-5)-5 x 10(-4) M using the DC, and P PP modes respectively, with a minimum detectability (S/N = 3) of 3 x 1 0(-7) M. The proposed method was successfully applied to the determina tion of ofloxacin in tablets and biological fluids. The results obtain ed were found to be in agreement with those obtained by a reference me thod. (C) 1998 Elsevier Science B.V. All rights reserved.