SIMULTANEOUS DETERMINATION OF PSEUDOEPHEDRINE HYDROCHLORIDE AND DIPHENHYDRAMINE HYDROCHLORIDE IN COUGH SYRUP BY GAS-CHROMATOGRAPHY (GC)

A simple, rapid and precise gas chromatographic method has been develo ped for the simultaneous determination of pseudoephedrine hydrochlorid e and diphenhydramine hydrochloride in cough syrup, using a SS column of 10% OV 1 on chromosorb W-HP (80-100 mesh) and nitrogen as a carrier gas at a flow rate of 30 ml min(-1). The oven temperature was program med at 135 degrees C for 1 min, with a rise of 10 degrees C min(-1) up to 250 degrees C (held for 5 min). The injector and detector port tem peratures were maintained at 280 degrees C. Detection was carried out using Flame ionization detector. Guaphenesin was used as an internal s tandard. Results of assay and recovery studies were statistically eval uated for its accuracy and precision. (C) 1998 Elsevier Science B.V. A ll rights reserved.