CHARACTERIZATION OF EXTRACELLULAR MENADION-CATALYZED H2O2 PRODUCTION BY NIH 3T3 CELLS/

Authors
YAMASHOJI S
Citation
S. Yamashoji, CHARACTERIZATION OF EXTRACELLULAR MENADION-CATALYZED H2O2 PRODUCTION BY NIH 3T3 CELLS/, Biochemistry and molecular biology international, 44(3), 1998, pp. 555-563
Citations number
24
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
44
Issue
3
Year of publication
1998
Pages
555 - 563
Database
ISI
SICI code
1039-9712(1998)44:3<555:COEMHP>2.0.ZU;2-4
Abstract
The extracellular menadione-catalyzed H2O2 production by NIH/3T3 cells was expected to depend on plasma membrane-bound NAD(P)H:quinone oxido reductase. This enzyme was estimated to be a flavoprotein with the mol ecular mass of 70 KDa. Km values of plasma membrane-bound NAD(P)H:quin one oxidoreductase producing H2O2 were 60 mu M for NADH and 150 mu M f or NADPH. Ca2+ ionophore A23187 controlled menadione-catalyzed H2O2 pr oduction by the cells in time-and concentration-dependent manner.