Retrotransposons propagate via an RNA intermediate which is then rever
se-transcribed and packaged into viruslike particles. They are either
copia-or gypsy like in coding domain order and sequence similarity, th
e gypsy-like elements sharing their organization with the retroviruses
but lacking retroviral envelope domains. Copia-like retrotransposons,
or at least their reverse transcriptase domains, appear broadly distr
ibuted in higher plants, but gypsy-like elements have been reported on
ly for scattered species. The authors have exploited the difference in
domain order between these groups to amplify and clone segments bridg
ing the reverse transcriptase - integrase region of specifically gypsy
-like retrotransposons. Species representative of the diversity of hig
her plants yielded products whose sequences establish that gypsy like
transposons are dispersed throughout the plant genomes. This class of
plant elements has been named romani retrotransposons. The presence of
both types ubiquitously in the fungi, plants and animals support thei
r existence as ancient distinct lineages and subsequent, vertical radi
ation.