A GENERAL-METHOD FOR GENE ISOLATION IN TAGGING APPROACHES - AMPLIFICATION OF INSERTION MUTAGENIZED SITES (AIMS)

Authors
FREY M STETTNER C GIERL A
Citation
M. Frey et al., A GENERAL-METHOD FOR GENE ISOLATION IN TAGGING APPROACHES - AMPLIFICATION OF INSERTION MUTAGENIZED SITES (AIMS), Plant journal, 13(5), 1998, pp. 717-721
Citations number
13
Categorie Soggetti
Plant Sciences
Journal title
Plant journal → ACNP
ISSN journal
09607412
Volume
13
Issue
5
Year of publication
1998
Pages
717 - 721
Database
ISI
SICI code
0960-7412(1998)13:5<717:AGFGII>2.0.ZU;2-G
Abstract
A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T-DNA tagging approaches has been developed. T he method can be generally applied and allows the rapid isolation of p utative gene sequences even in the presence of high numbers of inserti on sequences. The technique has been used successfully for the isolati on of the maize Bx1 gene tagged by a Mutator element.