M. Frey et al., A GENERAL-METHOD FOR GENE ISOLATION IN TAGGING APPROACHES - AMPLIFICATION OF INSERTION MUTAGENIZED SITES (AIMS), Plant journal, 13(5), 1998, pp. 717-721
A polymerase chain reaction (PCR) based procedure for the isolation of
genes in transposon or T-DNA tagging approaches has been developed. T
he method can be generally applied and allows the rapid isolation of p
utative gene sequences even in the presence of high numbers of inserti
on sequences. The technique has been used successfully for the isolati
on of the maize Bx1 gene tagged by a Mutator element.