THE EFFECT OF THE BLEACHING AGENT SODIUM PERBORATE ON MACROPHAGE ADHESION IN-VITRO - IMPLICATIONS IN EXTERNAL CERVICAL ROOT RESORPTION

The purpose of this study was to investigate the in vitro effect of so dium perborate, which is used as a bleaching agent in the treatment of discolored pulpless teeth, on substrate adherence capacity of macroph ages. Inflammatory macrophages were obtained from Wistar rats and resu spended in RPMI-1640 medium, As a test of macrophage adhesion, the adh erence capacity of macrophages to a plastic surface was determined, As says were conducted in Eppendorf tubes for 15 min of incubation at 37 degrees C in a humidified atmosphere of 5% CO2. The adherence index wa s calculated, Results showed that sodium perborate decreased in a dose -dependent manner and decreased significantly (p < 0.05) the adherence index of rat peritoneal macrophages, Sodium perborate was less potent than sodium hypochlorite and eugenol in inhibiting macrophage adhesio n, The inhibitory effect of sodium perborate on macrophage adhesion fu rther supports the concept that this agent is not implicated in extern al cervical root resorption associated with intracoronal bleaching.