CHARACTERIZATION OF MBOI SATELLITES IN CIRRHINA-MRIGALA AND CLARIAS-BATRACHUS (PISCES)

We have cloned and characterized two highly reiterated, tandemly repea ted, and A+T rich MboI DNA fragments, one in Cirrhina mrigala (Cyprini dae), with a monomer size of 266 bp, and one in Clarias batrachus (Cla riidae), with a monomer size of 227 bp. The MboI fragment in C. mrigal a is species-specific and absent in other carps, such as Catla catla a nd Labeo rohita. The MboI fragment in C. batrachus was also present in two other catfishes tested, namely Clarias gariepinus and Heteropneus tes fossilis. In C. mrigala x C. catla and C. mrigala x L. rohita hybr ids, the C. mrigala specific MboI fragment is inherited uniparentally. In the reciprocal hybrids of C. batrachus x H. fossilis, the satellit e ladder contains the bands of both parental species. The MboI satelli te of carp may be useful in genetic introgression analysis and that of catfish in distinguishing between gynogenetic progeny and true hybrid s.