COMPARATIVE-ANALYSIS OF MIB1 AND P53 EXPRESSION IN HUMAN BLADDER-TUMORS AND THEIR CORRELATION WITH CANCER PROGRESSION

Expression of p53 and MiB1, markers of tumor proliferation, was evalua ted in human bladder tumors, and correlated with ploidy and cancer pro gression in 83 consecutive patients. Transurethral resection ofa newly diagnosed bladder tumor was performed in 73 cases, and systematic bla dder biopsies were performed in 10 cases after bacillus Calmette-Gueri n (BCG) treatment. p53 and MiB1 expression were performed by an immuno histochemical technique and the ploidy was determined on a frozen frag ment of the tumor. p53 expression was correlated in relation to grade, stage and combination of grade and stage. MiB1 expression was correla ted with cytological grade, and a significant difference was demonstra ted between pT0 and pTa, pTa and pT1, pTa and pT2 tumors but not betwe en pT1 and greater than or equal to pT2 tumors. A discordance was obse rved for the comparison of p53 and MIBI values, stage by stage, sugges ting that these two techniques are independent of each other. A larger proportion of aneuploid tumors were positive for p53 and MiB1 (64.8 v s. 86.5%, respectively), but p53 and MiB1 immunostaining were not bett er indicators than ploidy alone to predict cancer progression.