ALTERATION OF TELOMERASE ACTIVITY ASSOCIATED WITH DEVELOPMENT AND EXTENSION OF EPITHELIAL OVARIAN-CANCER

Objective: To assess telomerase activity associated with the developme nt and extension of epithelial ovarian cancer and to investigate the r elationship between p53 gene status and telomerase activity. Methods: A total of 53 samples (41 epithelial ovarian cancers, five borderline epithelial tumors, four benign adenomas, and three surface epithelia) were examined for telomerase activity by the polymerase chain reaction (PCR)-based telomeric repeat amplification protocol assay. Mutations in the p53 gene were determined by PCR-single strand conformation poly morphism analysis. Results: Telomerase activity was detected in 33 of 41 epithelial ovarian cancers and in three of five borderline malignan cies but was not detectable in either benign tumors or normal surface epithelium. The mean (+/-standard deviation [SD]) intensity of telomer ase activity in cancers was significantly higher than that in borderli ne malignancies (10.6 +/- 8.2 versus 3.6 +/- 1.3). The positivity of t elomerase activity did not correlate with any clinical findings, but t he intensity (+/-SD) of telomerase activity was significantly higher i n tumors with lymph node involvement (12.2 +/- 8.3 versus 3.8 +/- 1.1) . Mutations of the p53 gene were observed in 44% of ovarian cancers; p 53 gene status did not relate to telomerase activity. Multivariate ana lysis showed that the intensity of telomerase activity was not an inde pendent factor for prognosis of patients with ovarian cancer. Conclusi on: Telomerase activity may be associated with development and extensi on of epithelial ovarian cancer. (C) 1998 by The American College of O bstetricians and Gynecologists.