We describe the construction of amplification primers designed to targ
et a portion of the mitochondrial cytochrome b locus in a variety of m
olluscan taxa. Combinations of two sets of primers successfully amplif
ied cytochrome b from several species of gastropod, bivalves, and ceph
alopods. Sequence analysis of these amplified products revealed nucleo
tide diversity in small samples within several of these taxa. We discu
ss the utility of these primer sets for studies of intraspecific phylo
geny in mollusks and potentially other invertebrates.