ASSIGNMENT OF PCR MARKERS TO RIVER BUFFALO CHROMOSOMES

In the process of developing a buffalo physical map using somatic cell hybrids and the cattle gene map as a template, ten PCR primers design ed for four coding genes: F10, FSHB, HBB, and CYM and six DNA segments : TGLA9, TGLA227, UWCA5, CSSM6, CSSM47 and RF131 were tested on a pane l of 47 buffalo-hamster somatic cell hybrids. F10-TGLA9, FSHB-HBB and UWCA5-TGLA227, respectively, were found to segregate together forming three syntenic groups. These three syntenic groups have also been repo rted in cattle, where they have been assigned to chromosomes BTA 12, B TA 15 and BTA 18, respectively Comparative mapping predicts the assign ment of these syntenic groups to river buffalo chromosomes BBU 13, BBU 16 and BBU 18, respectively. (C) Inra/Elsevier, Paris.