EFFECTS OF SOME ANTIGENIC FRACTIONS OF LEISHMANIA-MAJOR ON NITRIC-OXIDE PRODUCTION AND IL-12 SECRETION BY MURINE PERITONEAL-MACROPHAGES

Nitric oxide (NO) and IL-12 are important mediators of the immune resp onse to Leishmania major. In this study, the effects of L. major proma stigotes, crude antigenic fraction (CAF) and its subfractions on NO pr oduction and IL-12 secretion by BALB/c mice peritoneal macrophages is investigated. The subfractions of CAF, namely, fractions 1, 2 and 3, t hat were in the molecular weight range of 97.4-66, 66-45 and below 45 kDa, respectively, were separated by SDS-PAGE. NO production was deter mined by using Griess reagent and IL-12 was measured by ELISA. It was found that NO production was stimulated by promastigotes but not by CA F or its subfractions. IL-12 secretion was stimulated by promastigotes , CAF and fraction 1 while fractions 2 and 3 did not have any effect. (C) 1998 Elsevier Science B.V. All rights reserved.