Nitrogen elimination was investigated in two eutrophic Swiss lakes wit
h different hypolimnetic oxygen conditions, Nitrogen burial was estima
ted from sediment-trap and sediment-con studies. Denitrification and N
O3--ammonification rates were quantified with (NO3-)-N-15 and acetylen
e incubation experiments and whole-lake mass balances. The study confi
rmed earlier reports that the acetylene-block technique yields denitri
fication rates that Ne systematically too low. Denitrification rates o
btained from isotope tracer experiments were compatible with nitrogen
consumption rates observed in flux chamber experiments and whole-lake
mass balances. The NO3--ammonification contributed <5% to the NO3- con
sumption rate in Lake Baldegg. Coupled nitrification-denitrification s
eemed to be insignificant at the deepest station of Lake Baldegg. The
comparison of in situ denitrification rates measured at the deepest si
te (4.3 mmol m(-2) d(-1)) with the denitrification rate obtained from
whole-lake mass balances (6.1 mmol m(-2) d(-1)) indicates that enhance
d denitrification may be present in shallower sediments with a better
supply of O-2. Mass transfer coefficients for NO3- were similar in bot
h lakes (21.7 and 21.4 m yr(-1)). The NO3- concentration seems to be a
key parameter in determining denitrification rates.