Ba. Kulkarni et al., SDS-POLYACRYLAMIDE-GEL ELECTROPHORESIS OF SPERM PROTEINS OF BUFFALO AND CATTLE BULLS, Indian Journal of Animal Sciences, 67(2), 1997, pp. 110-112
Sperm protein patterns in buffalo and cattle breeding bulls were analy
sed by SDS-PAGE. Buffalo and cattle bull spermatozoa had 21 and 23 pro
tein bands of varying intensities and molecular weight ranging from >1
00 to 13 KDa. Distinct species differences in sperm protein patterns w
ere observed. A major protein of molecular weight 16 KDa and a broad p
rotein band having molecular weight below 14 KDa were present in cattl
e spermatozoa but absent in buffalo spermatozoa. Individual variations
and distinct breed differences in sperm protein patterns of Holstein-
Freisian. Jersey and crossbred cattle bulls and Murrah and Surti buffa
lo bulls were observed.