ENANTIOMERIC SEPARATION OF 4 METHYLENEDIOXYLATED AMPHETAMINES ON BETA-CYCLODEXTRIN CHIRAL STATIONARY PHASES

Native and derivatized beta-cyclodextrins such as chiral stationary ph ases (CSP) were used for the simultaneous enantiomeric separation of f our methylenedioxylated amphetamines (MDA, MDMA, MDEA and MBDB) by liq uid chromatography. Fluorimetric detection was used in order to enhanc e sensitivity and selectivity. The mobile phase was optimised by study ing the influence of pH, triethylamine concentration, organic solvent type, column temperature and flow rate of the mobile phase. This metho d was validated by determining linearity, precision, accuracy, limits of detection and quantification, and was applied to the stereoselectiv e analysis of illicit tablets (23 samples) and of human whole blood sa mples (spiked samples and two post-mortem cases). Whereas no significa nt deviation from a racemic ratio was observed in the tablets contents , the analysis of blood samples showed an enantioselective metabolism of MDMA.