EVALUATION OF SEROTONIN LEVELS IN HUMAN AQUEOUS-HUMOR

Purpose: Serotonin is biochemically present in the iris and ciliary bo dy of animals and humans. Controversial findings are reported about th e concentrations of serotonin in aqueous humor with respect to plasma in humans. The aim of this study was to evaluate the levels of seroton in both in aqueous humor and plasma in human subjects. Methods: In 50 patients with glaucoma or cataract, plasma and aqueous humor serotonin levels were measured by HPLC with electrochemical detection. Serotoni n plasma levels were also measured in 25 healthy subjects as controls. Results: In all patients with cataract or glaucoma, the aqueous humor serotonin concentration is significantly lower than that in plasma [1 .14+/-0.29 (SEM) vs. 5.33+/-1.03 ng/ml, p<0.01]. Furthermore, in the s ame patients and in 25 healthy controls, serotonin plasma levels were similar. Conclusion: Our study shows that serotonin is present in huma n aqueous humor and its concentration is 4 times lower than in plasma.