THE NEW APPROACHES TO ELECTROCHEMICAL INVESTIGATIONS OF BLOOD COMPONENTS

The differential linear sweep voltammetry technique is used to investi gate the effect of blood components (erythrocytes, leukocytes, plasma, lymphocyte fraction) on active intermediates of an oxygen reduction ( . OH, H2O2). Lymphocytes are shown to be most effective in the inhibit ion of radical intermediates of an oxygen reduction. The distinctions in the antiradical activity are found between intact lymphocytes of he althy mice and transformed lymphocytes of ascitic tumor (lymphoblastes ). These distinctions depend on cell concentrations and are clearly ob served at 2x10(4) ml(-1) or higher concentrations of lymphocytes in a chamber. The established peculiarities of the blood components' influe nce on oxygen intermediates can be used to elucidate the involvement o f the blood components into antioxidative protection mechanisms. (C) 1 998 Elsevier Science S.A.