KALEIDASEQ - A WEB-BASED TOOL TO MONITOR DATA-FLOW IN A HIGH-THROUGHPUT SEQUENCING FACILITY

Tracking data Flow in high throughput sequencing is important in maint aining a consistent number of successfully sequenced samples, making d ecisions on scheduling the flow of sequencing steps, resolving problem s at various steps and tracking the status of different projects. This is especially critical when the laboratory is handling a multitude of projects. We have built a Web-based data flow tracking package, calle d Kaleidaseq, which allows us to monitor the flow and quality of seque ncing samples through the steps of preparation of library plates, plaq ue-picking, preparation of templates, conducting sequencing reactions, loading of samples on gels, base-calling the traces, and calculating the quality of the sequenced samples. Kaleidaseq's suite of displays a llows for outstanding monitoring of the production sequencing process. The online display of current information that Kaleidaseq provides on both project status and process queues sorted by project enables accu rate real-time assessment of the necessary samples that must be proces sed to complete the project. This information allows the process manag er to allocate future resources optimally and schedule tasks according to scientific priorities. Quality of the sequenced samples can be tra cked on a daily basis, which allows the sequencing laboratory to maint ain a steady performance level and quickly resolve dips in quality. Ka leidaseq has a simple easy-to-use interface that allows access to all major functions and process queues from one Web page. This software pa ckage is modular and designed to allow additional processing steps and new monitoring variables to be added and tracked with ease. Access to the underlying relational database is through the Perl DBI interface, which allows for the use of different relational databases. Kaleidase q is available for Free use by the academic community from http://www. cshl.org/kaleidaseq.