SYNTHESIS OF HYDROPHOBIC PEPTIDES - AN FMOC SOLUBILIZING TAIL METHOD

The development of an Fmoc method for synthesis and purification of hy drophobic peptides using a ''solubilising tail'' strategy is described . Peptide-constructs of the form hydrophobic peptide-[4-Hmb ester]-sol ubilising peptide were synthesised. Procedures for forming the 4-Hmb e ster linkage, and sequences of solubilising peptides suitable for use with Fmoc SPPS, were investigated. The method was used to synthesise t wo model peptides and three designed transmembrane peptides. (C) 1998 Elsevier Science Ltd. All rights reserved.